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hek blue il 6 reporter line cells  (InvivoGen)


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    InvivoGen hek blue il 6 reporter line cells
    Hek Blue Il 6 Reporter Line Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hek+blue+il+6+reporter+cell+line/HEK-Blue+IL-6+cells/pm41145914-434-0-5
    Average 95 stars, based on 62 article reviews
    hek blue il 6 reporter line cells - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Optimisation of a screening platform for determining IL-6 inflammatory signalling in the senescence-associated secretory phenotype (SASP).
    Article Snippet: .. HEK-Blue IL-6 reporter cell line (InvivoGen, Cat. hkb-hil6) was cultured under identical conditions, with the addition of the HEKBlue selection antibiotics for maintenance of the transgene (InvivoGen, Cat. hb-sel), according to the manufacturer’s instructions. .. Conditioned medium was obtained from HF043 fibroblasts by replacing the cell culture medium with fresh supplemented DMEM (where relevant, containing the same amount of the compound of interest) and incubating for 24 h. Cell-based reporter assay Cell-based IL-6 measurement was initially carried out according to the manufacturer’s protocol (InvivoGen).

    Article Title: Optimisation of a screening platform for determining IL-6 inflammatory signalling in the senescence-associated secretory phenotype (SASP)
    Article Snippet: .. HEK-Blue IL-6 reporter cell line (InvivoGen, Cat. hkb-hil6) was cultured under identical conditions, with the addition of the HEK-Blue selection antibiotics for maintenance of the transgene (InvivoGen, Cat. hb-sel), according to the manufacturer’s instructions. ..

    Article Title: Small protein blockers of human IL-6 receptor alpha inhibit proliferation and migration of cancer cells
    Article Snippet: Goat anti-rabbit Abberior STAR RED was purchased from Abberior, Göttingen, Germany. .. HEK-Blue IL-6 reporter cell line (InvivoGen, San Diego, CA, USA) used in the study was cultured in Dulbecco’s modified Eagle’s medium (DMEM) (BioSera, Cholet, France) containing 2 mM L-glutamine and 4.5 g/l glucose, supplemented with 10% heat inactivated fetal bovine serum (FBS), and antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin (InvivoGen, San Diego, CA, USA), and HEK-Blue Selection (InvivoGen, San Diego, CA, USA)) at 37 °C in 5% CO 2 . ..

    Selection:

    Article Title: Optimisation of a screening platform for determining IL-6 inflammatory signalling in the senescence-associated secretory phenotype (SASP).
    Article Snippet: .. HEK-Blue IL-6 reporter cell line (InvivoGen, Cat. hkb-hil6) was cultured under identical conditions, with the addition of the HEKBlue selection antibiotics for maintenance of the transgene (InvivoGen, Cat. hb-sel), according to the manufacturer’s instructions. .. Conditioned medium was obtained from HF043 fibroblasts by replacing the cell culture medium with fresh supplemented DMEM (where relevant, containing the same amount of the compound of interest) and incubating for 24 h. Cell-based reporter assay Cell-based IL-6 measurement was initially carried out according to the manufacturer’s protocol (InvivoGen).

    Article Title: Optimisation of a screening platform for determining IL-6 inflammatory signalling in the senescence-associated secretory phenotype (SASP)
    Article Snippet: .. HEK-Blue IL-6 reporter cell line (InvivoGen, Cat. hkb-hil6) was cultured under identical conditions, with the addition of the HEK-Blue selection antibiotics for maintenance of the transgene (InvivoGen, Cat. hb-sel), according to the manufacturer’s instructions. ..

    Article Title: Small protein blockers of human IL-6 receptor alpha inhibit proliferation and migration of cancer cells
    Article Snippet: Goat anti-rabbit Abberior STAR RED was purchased from Abberior, Göttingen, Germany. .. HEK-Blue IL-6 reporter cell line (InvivoGen, San Diego, CA, USA) used in the study was cultured in Dulbecco’s modified Eagle’s medium (DMEM) (BioSera, Cholet, France) containing 2 mM L-glutamine and 4.5 g/l glucose, supplemented with 10% heat inactivated fetal bovine serum (FBS), and antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin (InvivoGen, San Diego, CA, USA), and HEK-Blue Selection (InvivoGen, San Diego, CA, USA)) at 37 °C in 5% CO 2 . ..

    Modification:

    Article Title: Small protein blockers of human IL-6 receptor alpha inhibit proliferation and migration of cancer cells
    Article Snippet: Goat anti-rabbit Abberior STAR RED was purchased from Abberior, Göttingen, Germany. .. HEK-Blue IL-6 reporter cell line (InvivoGen, San Diego, CA, USA) used in the study was cultured in Dulbecco’s modified Eagle’s medium (DMEM) (BioSera, Cholet, France) containing 2 mM L-glutamine and 4.5 g/l glucose, supplemented with 10% heat inactivated fetal bovine serum (FBS), and antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin (InvivoGen, San Diego, CA, USA), and HEK-Blue Selection (InvivoGen, San Diego, CA, USA)) at 37 °C in 5% CO 2 . ..



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    ( A ) Pull-downs with native and denatured (heat-inactivated and carbamidomethylated) C-terminally His-tagged C3-LHF1 were performed on human kidney homogenate. ( B ) Significantly enriched (BH-adj. t-test p -value < 0.05, log 2 FC > 1) proteins in the kidney pull-downs included the <t>IL6</t> signaling component IL6ST (gp130). ( C ) Thermal proteome profiling by proteome integral solubility alteration assay (TPP-PISA) in human kidney lysates to identify putative C3-LHF1 interacting proteins ( n = 3 technical replicates). Only sign. altered (BH-adj. t-test p -value < 0.05, log 2 FC > 0.58) and plasma membrane-resident proteins are displayed after addition of 1 or 10 µM C3-LHF1 (1 h, 37 °C). Proteins stabilized by interactions with C3-LHF1 display a higher abundance in the treated samples (IL6ST depicted in red). ( D ) C3-LHF1 effect on IL6R/IL6ST signaling was tested in a HEK-Blue IL6Rα/IL6ST reporter line (incubation o/N at 37 °C). C3-LHF1 effect was concentration dependent, and activating, unlike bulk C3 ( n = 4, mean ± SE; two-sided t-test p -value < 0.001 = *; IL6 used at 3 × 10 −6 µg/mL, p -value vs. untreated control: 0.0000001; p -values for 5-10-20 µM C3-LHF1 vs. untreated control: 0.000119, 0.000009, and 0.000006, respectively). ( E ) In HEK-Blue IL6Rα/IL6ST cell line with both IL6 and C3-LHF1 (o/N at 37 °C), a partial competition can be observed at IL6 concentrations of >1 × 10 −4 µg/mL ( n = 4, mean ± SE). ( F ) Tocilizumab (TOC, 2 µg/mL for 3 h at 37 °C) did not inhibit the C3-LHF1 response in the IL6Rα/IL6ST cell line, unlike the IL6 response at 1 × 10 −4 µg/mL ( n = 4, mean ± SE, two-sided t-test p -value < 0.001 = *; p -value IL6 + tocilizumab vs. IL6 at 1 × 10 −4 µg/mL = 1.5 × 10 −9 ). .
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    ( A ) Pull-downs with native and denatured (heat-inactivated and carbamidomethylated) C-terminally His-tagged C3-LHF1 were performed on human kidney homogenate. ( B ) Significantly enriched (BH-adj. t-test p -value < 0.05, log 2 FC > 1) proteins in the kidney pull-downs included the <t>IL6</t> signaling component IL6ST (gp130). ( C ) Thermal proteome profiling by proteome integral solubility alteration assay (TPP-PISA) in human kidney lysates to identify putative C3-LHF1 interacting proteins ( n = 3 technical replicates). Only sign. altered (BH-adj. t-test p -value < 0.05, log 2 FC > 0.58) and plasma membrane-resident proteins are displayed after addition of 1 or 10 µM C3-LHF1 (1 h, 37 °C). Proteins stabilized by interactions with C3-LHF1 display a higher abundance in the treated samples (IL6ST depicted in red). ( D ) C3-LHF1 effect on IL6R/IL6ST signaling was tested in a HEK-Blue IL6Rα/IL6ST reporter line (incubation o/N at 37 °C). C3-LHF1 effect was concentration dependent, and activating, unlike bulk C3 ( n = 4, mean ± SE; two-sided t-test p -value < 0.001 = *; IL6 used at 3 × 10 −6 µg/mL, p -value vs. untreated control: 0.0000001; p -values for 5-10-20 µM C3-LHF1 vs. untreated control: 0.000119, 0.000009, and 0.000006, respectively). ( E ) In HEK-Blue IL6Rα/IL6ST cell line with both IL6 and C3-LHF1 (o/N at 37 °C), a partial competition can be observed at IL6 concentrations of >1 × 10 −4 µg/mL ( n = 4, mean ± SE). ( F ) Tocilizumab (TOC, 2 µg/mL for 3 h at 37 °C) did not inhibit the C3-LHF1 response in the IL6Rα/IL6ST cell line, unlike the IL6 response at 1 × 10 −4 µg/mL ( n = 4, mean ± SE, two-sided t-test p -value < 0.001 = *; p -value IL6 + tocilizumab vs. IL6 at 1 × 10 −4 µg/mL = 1.5 × 10 −9 ). .
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    ( A ) Pull-downs with native and denatured (heat-inactivated and carbamidomethylated) C-terminally His-tagged C3-LHF1 were performed on human kidney homogenate. ( B ) Significantly enriched (BH-adj. t-test p -value < 0.05, log 2 FC > 1) proteins in the kidney pull-downs included the <t>IL6</t> signaling component IL6ST (gp130). ( C ) Thermal proteome profiling by proteome integral solubility alteration assay (TPP-PISA) in human kidney lysates to identify putative C3-LHF1 interacting proteins ( n = 3 technical replicates). Only sign. altered (BH-adj. t-test p -value < 0.05, log 2 FC > 0.58) and plasma membrane-resident proteins are displayed after addition of 1 or 10 µM C3-LHF1 (1 h, 37 °C). Proteins stabilized by interactions with C3-LHF1 display a higher abundance in the treated samples (IL6ST depicted in red). ( D ) C3-LHF1 effect on IL6R/IL6ST signaling was tested in a HEK-Blue IL6Rα/IL6ST reporter line (incubation o/N at 37 °C). C3-LHF1 effect was concentration dependent, and activating, unlike bulk C3 ( n = 4, mean ± SE; two-sided t-test p -value < 0.001 = *; IL6 used at 3 × 10 −6 µg/mL, p -value vs. untreated control: 0.0000001; p -values for 5-10-20 µM C3-LHF1 vs. untreated control: 0.000119, 0.000009, and 0.000006, respectively). ( E ) In HEK-Blue IL6Rα/IL6ST cell line with both IL6 and C3-LHF1 (o/N at 37 °C), a partial competition can be observed at IL6 concentrations of >1 × 10 −4 µg/mL ( n = 4, mean ± SE). ( F ) Tocilizumab (TOC, 2 µg/mL for 3 h at 37 °C) did not inhibit the C3-LHF1 response in the IL6Rα/IL6ST cell line, unlike the IL6 response at 1 × 10 −4 µg/mL ( n = 4, mean ± SE, two-sided t-test p -value < 0.001 = *; p -value IL6 + tocilizumab vs. IL6 at 1 × 10 −4 µg/mL = 1.5 × 10 −9 ). .
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    ( A ) Pull-downs with native and denatured (heat-inactivated and carbamidomethylated) C-terminally His-tagged C3-LHF1 were performed on human kidney homogenate. ( B ) Significantly enriched (BH-adj. t-test p -value < 0.05, log 2 FC > 1) proteins in the kidney pull-downs included the <t>IL6</t> signaling component IL6ST (gp130). ( C ) Thermal proteome profiling by proteome integral solubility alteration assay (TPP-PISA) in human kidney lysates to identify putative C3-LHF1 interacting proteins ( n = 3 technical replicates). Only sign. altered (BH-adj. t-test p -value < 0.05, log 2 FC > 0.58) and plasma membrane-resident proteins are displayed after addition of 1 or 10 µM C3-LHF1 (1 h, 37 °C). Proteins stabilized by interactions with C3-LHF1 display a higher abundance in the treated samples (IL6ST depicted in red). ( D ) C3-LHF1 effect on IL6R/IL6ST signaling was tested in a HEK-Blue IL6Rα/IL6ST reporter line (incubation o/N at 37 °C). C3-LHF1 effect was concentration dependent, and activating, unlike bulk C3 ( n = 4, mean ± SE; two-sided t-test p -value < 0.001 = *; IL6 used at 3 × 10 −6 µg/mL, p -value vs. untreated control: 0.0000001; p -values for 5-10-20 µM C3-LHF1 vs. untreated control: 0.000119, 0.000009, and 0.000006, respectively). ( E ) In HEK-Blue IL6Rα/IL6ST cell line with both IL6 and C3-LHF1 (o/N at 37 °C), a partial competition can be observed at IL6 concentrations of >1 × 10 −4 µg/mL ( n = 4, mean ± SE). ( F ) Tocilizumab (TOC, 2 µg/mL for 3 h at 37 °C) did not inhibit the C3-LHF1 response in the IL6Rα/IL6ST cell line, unlike the IL6 response at 1 × 10 −4 µg/mL ( n = 4, mean ± SE, two-sided t-test p -value < 0.001 = *; p -value IL6 + tocilizumab vs. IL6 at 1 × 10 −4 µg/mL = 1.5 × 10 −9 ). .
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    Image Search Results


    ( A ) Pull-downs with native and denatured (heat-inactivated and carbamidomethylated) C-terminally His-tagged C3-LHF1 were performed on human kidney homogenate. ( B ) Significantly enriched (BH-adj. t-test p -value < 0.05, log 2 FC > 1) proteins in the kidney pull-downs included the IL6 signaling component IL6ST (gp130). ( C ) Thermal proteome profiling by proteome integral solubility alteration assay (TPP-PISA) in human kidney lysates to identify putative C3-LHF1 interacting proteins ( n = 3 technical replicates). Only sign. altered (BH-adj. t-test p -value < 0.05, log 2 FC > 0.58) and plasma membrane-resident proteins are displayed after addition of 1 or 10 µM C3-LHF1 (1 h, 37 °C). Proteins stabilized by interactions with C3-LHF1 display a higher abundance in the treated samples (IL6ST depicted in red). ( D ) C3-LHF1 effect on IL6R/IL6ST signaling was tested in a HEK-Blue IL6Rα/IL6ST reporter line (incubation o/N at 37 °C). C3-LHF1 effect was concentration dependent, and activating, unlike bulk C3 ( n = 4, mean ± SE; two-sided t-test p -value < 0.001 = *; IL6 used at 3 × 10 −6 µg/mL, p -value vs. untreated control: 0.0000001; p -values for 5-10-20 µM C3-LHF1 vs. untreated control: 0.000119, 0.000009, and 0.000006, respectively). ( E ) In HEK-Blue IL6Rα/IL6ST cell line with both IL6 and C3-LHF1 (o/N at 37 °C), a partial competition can be observed at IL6 concentrations of >1 × 10 −4 µg/mL ( n = 4, mean ± SE). ( F ) Tocilizumab (TOC, 2 µg/mL for 3 h at 37 °C) did not inhibit the C3-LHF1 response in the IL6Rα/IL6ST cell line, unlike the IL6 response at 1 × 10 −4 µg/mL ( n = 4, mean ± SE, two-sided t-test p -value < 0.001 = *; p -value IL6 + tocilizumab vs. IL6 at 1 × 10 −4 µg/mL = 1.5 × 10 −9 ). .

    Journal: The EMBO Journal

    Article Title: Proteolytic profiling of human plasma reveals an immunoactive complement C3 fragment

    doi: 10.1038/s44318-025-00598-8

    Figure Lengend Snippet: ( A ) Pull-downs with native and denatured (heat-inactivated and carbamidomethylated) C-terminally His-tagged C3-LHF1 were performed on human kidney homogenate. ( B ) Significantly enriched (BH-adj. t-test p -value < 0.05, log 2 FC > 1) proteins in the kidney pull-downs included the IL6 signaling component IL6ST (gp130). ( C ) Thermal proteome profiling by proteome integral solubility alteration assay (TPP-PISA) in human kidney lysates to identify putative C3-LHF1 interacting proteins ( n = 3 technical replicates). Only sign. altered (BH-adj. t-test p -value < 0.05, log 2 FC > 0.58) and plasma membrane-resident proteins are displayed after addition of 1 or 10 µM C3-LHF1 (1 h, 37 °C). Proteins stabilized by interactions with C3-LHF1 display a higher abundance in the treated samples (IL6ST depicted in red). ( D ) C3-LHF1 effect on IL6R/IL6ST signaling was tested in a HEK-Blue IL6Rα/IL6ST reporter line (incubation o/N at 37 °C). C3-LHF1 effect was concentration dependent, and activating, unlike bulk C3 ( n = 4, mean ± SE; two-sided t-test p -value < 0.001 = *; IL6 used at 3 × 10 −6 µg/mL, p -value vs. untreated control: 0.0000001; p -values for 5-10-20 µM C3-LHF1 vs. untreated control: 0.000119, 0.000009, and 0.000006, respectively). ( E ) In HEK-Blue IL6Rα/IL6ST cell line with both IL6 and C3-LHF1 (o/N at 37 °C), a partial competition can be observed at IL6 concentrations of >1 × 10 −4 µg/mL ( n = 4, mean ± SE). ( F ) Tocilizumab (TOC, 2 µg/mL for 3 h at 37 °C) did not inhibit the C3-LHF1 response in the IL6Rα/IL6ST cell line, unlike the IL6 response at 1 × 10 −4 µg/mL ( n = 4, mean ± SE, two-sided t-test p -value < 0.001 = *; p -value IL6 + tocilizumab vs. IL6 at 1 × 10 −4 µg/mL = 1.5 × 10 −9 ). .

    Article Snippet: HEK-Blue IL6 reporter line , Invivogen , #hkb-hil6.

    Techniques: Solubility, Clinical Proteomics, Membrane, Incubation, Concentration Assay, Control